Abstract Background and Aims A recent meta-analysis highlighted the impact of different types phosphate binders on vitamin D metabolism in patients with stage 3 CKD. Sevelamer was associated lower serum 25(OH) levels probably due to reduced intestinal absorption D3 (cholecalciferol). Corresponding effects hemodialysis have not been studied. Method We performed an observational prospective study...

In this study, a dispersive liquid–liquid microextraction method using an extraction solvent lighter than water has been developed for the extraction and preconcentration of cholecalciferol and calcifediol from plasma samples followed by high performance liquid chromatography determination. Initially, acetonitrile and sodium chloride (NaCl) are added into the plasma as an extraction solvent and...