b a ckground: anoph eles culicifacies, a major malarial vector has been recognized as a complex of five sibling species, a, b, c, d and e. these sibling species exhibit varied vectorial capacity, host specificity and susceptibility to malarial parasites/ insecticides. in this study, a pcr assay developed earlier for distinguishing the five individual species was validated on samples of a n . cu...

BACKGROUND Anopheles culicifacies, a major malarial vector has been recognized as a complex of five sibling species, A, B, C, D and E. These sibling species exhibit varied vectorial capacity, host specificity and susceptibility to malarial parasites/ insecticides. In this study, a PCR assay developed earlier for distinguishing the five individual species was validated on samples of An. culicifa...

To aid in identifying key predators of Proconiini sharpshooter species present in California, we developed and tested molecular diagnostic markers for the glassy-winged sharpshooter, Homalodisca coagulata (Say), and smoke-tree sharpshooter, Homalodisca liturata (Ball) (Homoptera: Cicadellidae). Two different types of markers were compared, those targeting single-copy sequence characterized ampl...

The Leishmania tarentolae cytochrome oxidase (EC 1.9.3.1) subunit II (COII) and maxicircle unidentified reading frame 3 (MURF3) mRNAs are edited internally by the addition of four and five uridine residues, respectively, which eliminate -1 and +1 reading frameshifts in the gene sequences. The editing events in COII are conserved in three kinetoplastid species, and those in MURF3 are conserved i...

BACKGROUND The mitochondrial gene COI has been widely used by taxonomists as a standard DNA barcode sequence for the identification of many animal species. However, the COI region is of limited use for identifying certain species and is not efficiently amplified by PCR in all animal taxa. To evaluate the utility of COI as a DNA barcode and to identify other barcode genes, we chose the aphid sub...

Antibodies against synthetic peptides derived from the DNA sequence of human cytochrome c oxidase subunit II (COII) have been tested for their capacity to immunoprecipitate the whole enzyme complex. Antibodies against the COOH-terminal undecapeptide of COII (anti-COII-C), when incubated with a Triton X-100 mitochondrial lysate from HeLa cells pulse-labeled with [35S]methionine under conditions ...

Nuclear genetic mutations have been extensively investigated in solid tumors. However, the role of the mitochondrial genome remains uncertain. Since the metabolism of solid tumors is associated with aerobic glycolysis and high lactate production, tumors may have mitochondrial dysfunctions. Familial adenomatous polyposis (FAP) is a rare form‌ of colorectal cancer and an autosomal dominant inheri...

Human mitochondrial DNAs (mtDNAs) from 153 independent samples encompassing seven Asian populations were surveyed for sequence variation using the polymerase chain reaction (PCR), restriction endonuclease analysis and oligonucleotide hybridization. All Asian populations were found to share two ancient AluI/DdeI polymorphisms at nps 10394 and 10397 and to be genetically similar indicating that t...

Experimental work pertaining to the molecular cytogenetics of malaria vector species of mosquitoes by the application of PCR technique has been carried out. The main objectives of the study included the sequence characterization of nuclear rDNA internal transcribed spacers 1 and 2 (ITS1, ITS2) and mitochondrial DNA COII gene as potential molecular markers for studying genetic relatedness and ph...

Four dinuclear CoII complexes have been synthesized and structurally characterized with the dinucleating ligand susan (susan=4,7-dimethyl-1,1,10,10-tetra(2-pyridylmethyl)-1,4,7,10-tetraazadecane) varying in exogeneous ligands: [(susan){CoII(CH3CN)2}2](PF6)4, [(susan){CoIICl}2](ClO4)2, [(susan){CoIIBr}2](ClO4)2, [(susan){CoII(μ-OH)CoII}](ClO4)3. The ions are six-coordinate CH3CN ligands five-coo...