Microscopy has incited many of the greatest revolutions in scientific history. In particular, these inventions have sparked paradigm shifts in biology, and discoveries like the structure of DNA are indebted to such studies. The development of the field spans from recent work like scanning electron microscopy and atomic force microscopy to the original optical microscope, which dates back hundre...

For centuries, lens-based microscopy, such as light, phase-contrast, fluorescence, confocal and electron microscopy, has played an important role in the evolution of modern science and technology. In 1999, a novel form of microscopy, known as coherent diffraction imaging (CDI) or lensless imaging, was developed and transformed our traditional view of microscopy, in which the diffraction pattern...

Diffraction imaging in the far-field can detect 3D morphological features of an object for its coherent nature. We describe methods for accurate calculation and analysis of diffraction images of scatterers of single and double spheres by an imaging unit based on microscope objective at non-conjugate positions. A quantitative study of the calculated diffraction imaging in spectral domain has bee...

We report the development of a novel experimental chamber for experiments in soft x-ray diffraction tomography, diffraction imaging of single biological objects, and magnetic speckle imaging. The chamber will allow for acquisition of nearly full three-dimensional diffraction data sets as well as high magnification zone plate images of holograms for the diffraction tomography experiment.

Diffraction imaging of scattered light allows extraction of information on scatterer's morphology. We present a method for accurate simulation of diffraction imaging of single particles by combining rigorous light scattering model with ray-tracing software. The new method has been validated by comparison to measured images of single microspheres. Dependence of fringe patterns on translation of ...