echinacea purpurea is an herbaceous perennial plant as members of the asteraceae family. it is one the important medicinal plant in pharmacy industrial. active substances of echinaceae are amplifier of body's immune system and antivirus. echinacea is not native to iran. the phytochemical traits of medicinal plants depend on ecological conditions involving growing areas, climate conditions; vari...

Extracts of the plant Echinacea purpurea are widely used for medicinal purposes. Effective quality control of these extracts requires rapid methods to determine their chemical composition. A new method for analysis of caffeic acid derivatives and alkamides from Echinacea extracts has been developed. With this method, isomeric isobutylamides and 2-methylbutylamides can be distinguished, a capabi...

A new, rapid, and reproducible reversed-phased liquid chromatography (LC) method with ultraviolet (UV) absorption and/or mass spectrometry (MS) detection has been developed and validated for quantitation of cichoric acid, a major constituent of Echinacea spp. The method involves the use of a short Phenomenex Hydro-RP C18 column (4 microm, 50 mm x 3.0 mm id) and a simple isocratic mobile phase p...

Echinacea purpurea (L.) Moench, Asteraceae, a native of North America is among the most frequently utilized Echinacea spp. for medicinal, cosmetic, and beverage preparations. Most of the echinacea preparations in the industry originate from wild harvesting and few from non-standardized (unregulated) cultivation and harvesting. According to the recent reports (Brevoort 1996), echinacea preparati...

Echinacea preparations are widely used herbal medicines for the prevention and treatment of colds and minor infections. There is little evidence for the individual components in Echinacea that contribute to immune regulatory activity. Activity of an ethanolic Echinacea extract and several constituents, including cichoric acid, have been examined using three in vitro measures of macrophage immun...

background: purple coneflower (echinacea purpurea l.) is one of the most important medicinal plants. hairy root culture of echinace, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. objective: the present study investigates the effects of kno3, cacl2 and mgso4 concentrations on growth and the biosynthesis of cichoric acid in echinacea ...

Background: Purple coneflower (Echinacea purpurea L.) is one of the most important medicinal plants. Hairy root culture of Echinace, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. Objective: The present study investigates the effects of KNO3, CaCl2 and MgSO4 concentrations on growth and the biosynthesis of cichoric acid in Echinacea...

Echinacea species are used for beneficial effects on immune function, and various prevalent phytochemicals have immunomodulatory effects. Using a commercial E. purpurea (L.) Moench product, we have evaluated the myelopoietic effect on bone marrow of rats treated with various extracts and correlated this with their chemical class composition. Granulocyte/macrophage-colony forming cells (GM-CFCs)...

This is the first report to identify the presence of chicoric acid (cichoric acid; also known as dicaffeoyltartaric acid, which is a caffeic acid derivatizedwith tartaric acid) in basil leaves. Rosmarinic acid, chicoric acid and caftaric acid (in the order of most abundant to least; all derivatives of caffeic acid) were identified in fresh basil leaves. Rosmarinic acid was themain phenolic comp...

The aim of this study was to set up and validate an RP-LC method with DAD-detection to quantify caffeic acid derivatives in various Echinacea spp. Samples were extracted with 80% methanol. The analyses were carried out on a Lichrospher RP-18 column (125 mm x 4 mm i.d., 5 microm), with a mobile phase gradient, which increases the acetonitrile level in a phosphoric acid solution (0.1%). The flow ...