Background: Hepatitis C virus (HCV) causes acute and chronic human hepatitis infections. Due to the high genetic diversity and high rates of mutations in the genetic material so far there is no approved vaccine against HCV. Materials and Methods: The aim of this study was to determination B and T cell conserved epitopes of E1 and E2 proteins from HCV and construction of a chimeric pepti...

Hepatitis C virus (HCV) is responsible for a vast majority of liver failure cases. HCV is a kind of blood disease appraised to chronically infect 3% of the worlds’ population causing significant morbidity and mortality. Therefore, a complete knowledge of humoral responses against HCV, resulting antibodies, and virus-receptor and virus-antibody interactions, are essential to design a vaccine. HC...

the object of this cross sectional study was to determine the hcv subtype 3a envelope protein binding affinity with human leukocyte antigen. envelope 1 (e1) protein is one of the structural proteins responsible for entering the cells through the receptors. the binding affinity of e1 protein epitopes to the selected human leukocyte antigen (hla) class i alleles was investigated using the compute...

The hepatitis C virus E1 and E2 envelope proteins are targeted to the endoplasmic reticulum, but instead of being secreted, they are retained in a pre-Golgi compartment, at least partly in a misfolded state. Since secretory proteins which are retained in the endoplasmic reticulum frequently can activate the transcription of intraluminal chaperone proteins, we measured the effect of the E1 and E...

conclusions the comprehensive analysis of a protein with important roles has never been easy, and in case of e2 envelope glycoprotein of hgv, there is no much data on its molecular and immunological features, clinical significance and its pathogenic potential in hepatitis or any other gbv-c related diseases. so, results of the present study may explain some structural, physiological and immunol...

conclusions these findings of the present study indicate that the recombinant coree1e2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential hcv vaccine candidate. results in our study, the virus-like particle of rcoree1e2 with 70 nm size, was shown by electron microscopy and proved the self-assembly in vitro in a yeast expression system. materials and methods by ...

The hepatitis B virus (HBV) envelope protein (S) self-assembles into subviral particles used as commercial vaccines against hepatitis B. These particles are excellent carriers for foreign epitopes, which can be inserted into the external hydrophilic loop or at the N- or C-terminal end of the HBV S protein. We show here that the N-terminal transmembrane domain (TMD) of HBV S can be replaced by t...

The Hepatitis C virus E1 and E2 envelope proteins are the major players in all events required for virus entry into target cells. In addition, the recently developed HCV cell culture system has indicated that E1E2 heterodimer formation is a prerequisite for viral particle production. In this paper, we explored a new genetic approach to construct intergenotypic 2a/1b chimeras, maintaining the st...

Various strategies involving the use of hepatitis C virus (HCV) E1 and E2 envelope glycoproteins as immunogens have been developed for prophylactic vaccination against HCV. However, the ideal mode of processing and presenting these immunogens for effective vaccination has yet to be determined. We used our recently described vaccine candidate based on full-length HCV E1 or E2 glycoproteins fused...

results the present work figured out that the heterogeneity of the quasispecies of our local strains 4a was high showing up 15% diversity. this study also showed four glycosylation sites that play an important role in the entry of the virus and protein folding. besides, different epitpoes were identified in different regions of the e1 and e2 domains; a finding which would help in determining th...