background: in vitro maturation (ivm) is a promising treatment option for certain infertile women. nowadays, with the aid of polscope, it has become possible to evaluate zona pellucida (zp) characteristics as a parameter of oocyte quality. moreover, quality of oocytes can be influenced by many factors, such as patient’s age. the polscope system is a non-invasive technique to assess birefringent...

background: in vitro maturation (ivm) of immature oocytes collected from ovary has been proposed for fertility preservation. in addition, quality of oocytes post ivm is one of the factors determining its developmental competence. by using the non-invasive polscope system, both meiotic spindle (ms) and zona pellucida (zp) can be assessed in living oocytes. objective: the aim was to investigate t...

Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...

background: the cryopreservation of human oocyte would make a significant contribution to infertility treatment, such as using it for oocyte donation and for patients a bout to lose ovarian function due to surgery or chemotherapy. despite of using standard freezing straws and cryovials or even open pulled straws, only a few successful pregnancies have been arisen from cryopreserved human oocyte...

Background: Before human MII oocytes are vitrified they are usually denuded from their cumulus cells. In this study we wanted to investigate the effects of an intact corona radiata on the vitrification and fertilization of human oocytes. Materials and Methods: The study comprised two different parts. In Part 1, 36 MII stage oocytes, from 6 patients, were randomly assigned into a control group, ...

In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...