Chemical probes that target classes of proteins based on shared functional properties have emerged as powerful tools for proteomics. The metabolome rivals, if not surpasses, the proteome in terms of size and complexity, suggesting that efforts to profile metabolites would also benefit from targeted technologies. Here we apply the principle of chemoselective probes to the metabolome, creating a ...

BACKGROUND Tropical rainforests are frequently limited by soil nutrient availability. However, the response of the metabolic phenotypic plasticity of trees to an increase of soil nutrient availabilities is poorly understood. We expected that increases in the ability of a nutrient that limits some plant processes should be detected by corresponding changes in plant metabolome profile related to ...

An organism’s metabolome is the cataloguing of the entire set of metabolites found in the organism. It is dynamic and thus just a snapshot of the processes going on in the organism at one specific time point. When combined with other ‘‘omics’’ data, a more complete understanding of the organism emerges (Fiehn et al., 2000). However, several technical challenges to obtaining a metabolomic profil...

An organism’s metabolome is the cataloguing of the entire set of metabolites found in the organism. It is dynamic and thus just a snapshot of the processes going on in the organism at one specific time point. When combined with other ‘‘omics’’ data, a more complete understanding of the organism emerges (Fiehn et al., 2000). However, several technical challenges to obtaining a metabolomic profil...

BACKGROUND Although the effects of acute dietary interventions on the human metabolome have been studied, the extent to which the metabolome can be normalized by extended dietary standardization has not yet been examined. OBJECTIVE We examined the metabolic profiles of healthy human subjects after extended dietary standardization to see whether the inherent variation in the human metabolome c...

An ongoing challenge in biology is to predict the phenotypes of individuals from their genotypes. Genetic variants that cause disease often change an individual's total metabolite profile, or metabolome. In light of our extensive knowledge of metabolic pathways, genetic variants that alter the metabolome may help predict novel phenotypes. To link genetic variants to changes in the metabolome, w...

Metabolomics is a technique that allows for the evaluation of entire extractable chemical profile plant, example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due drought. Metabolomic analysis dependent upon efficiency extraction protocol. Currently, there are two common procedures widely in metabolomic experiments, extract fro...