Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S. hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The 18SrRNA gene as a specific target ...

abstract breast cancer is one of the major health problems of the eastern world. regardless of the survival rate improvement with progression in screening and adjuvant systemic therapies, still one – third of the patients with primary breast cancer have recurrence of micro metastasis after 10 years.  it is important to discover a reliable biomarker for detection of breast cancer. the underlying...

developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...

In order to determine the fumonisin producingisolates of the Fusarium fujikuroi species complex (FFSC), associated with infected spikes, and ear rot of grass plants, we examined 78 samples collected from Kermanshah province, Iran. Based on morphological characters, twenty two isolates assigned to the FFSC and were identified as F. proliferatum (ten isolates), F. verticillioides (seven isolates)...

Mycoplasma gallisepticum (MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections.This study was carried out for molecular detection and phylogenetic analysis of MG infections in commercial and backyard turkey flocks ...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...