BACKGROUND The increasing use of zebrafish model has not been accompanied by the evolution of proper anaesthesia for this species in research. The most used anaesthetic in fishes, MS222, may induce aversion, reduction of heart rate, and consequently high mortality, especially during long exposures. Therefore, we aim to explore new anaesthetic protocols to be used in zebrafish by studying the qu...

in this study the effects of the most popular anaesthetics including: ms222, clove oil (eugenia caryophyllata) and 2-phenoxyethanol, on some haematological and serum enzymes of cyprinus carpio were investigated. firstly the effective dose of each anaesthetics (3 minutes for anesthesia and 5 minutes for recovery ) and lc50 of them were calculated in c. carpio. fish were anaesthetized with the ef...

  There is a few available information about the effect of anesthetics, specially electroanesthesia on immune parameters in fish. In the present work, two anesthetics, MS222 (50 ppm), clove oil (25 ppm), and electroanesthesia were tested in rainbow trout (Oncorhynchus mykiss) in narcosis stage. The results showed, clove oil and electroanesthesia increase the lysozyme activity 24 h after anesthe...

  There is a few available information about the effect of anesthetics, specially electroanesthesia on immune parameters in fish. In the present work, two anesthetics, MS222 (50 ppm), clove oil (25 ppm), and electroanesthesia were tested in rainbow trout (Oncorhynchus mykiss) in narcosis stage. The results showed, clove oil and electroanesthesia increase the lysozyme activity 24 h after anesthe...

The present study aimed to determine the optimal concentration of three anaesthetics, name MS222, ketamine, and tobacco extract, in grass carp juveniles (107.78±22.11 g body weight and 226.74±13.06 mm total body length). Treatments were as follows: 100 mg L-1 of MS222 (control group), 5.5, 8, 11, 18.5, and 22 mg L-1 of ketamine, and 10, 13.33, 20, 26.27, and 40 mg L-1 of tobacco extract. In add...

BACKGROUND Zebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, continuously, cardiac ventricle function in 2-5 days old zebrafish embryos embedded in 0.6% agar and examined under light microscopy at room temperature (22 degrees C). Using vi...