in this study, in vitro non-symbiotic germination of self pollinated seeds, protocorms survival percentage and protocorms growth of phalaenopsis ‘kyoto’ were investigated. for this purpose, five different modified media include vasin and went (vw), murashing and skoog (ms), ½ ms, knudson c (kc), fast that supplemented with two g/l peptone, 15 percent sterile coconut water or two g/l peptone tog...

Silymarin production by hairy root culture of Milk thistle (Silybum marianum L. Gaertn) was investigated using Agrobacterium rhizogenes AR15834. Hairy roots were induced by injection or inoculation of explants with A. rhizogenes. One month old hairy roots were dissected from the explants and grown in Murashing and Skoog (MS) liquid medium. Polymerase Chain Reaction (PCR) using the B gene and th...

silymarin production by hairy root culture of milk thistle (silybum marianum l. gaertn) was investigated using agrobacterium rhizogenes ar15834. hairy roots were induced by injection or inoculation of explants with a. rhizogenes. one month old hairy roots were dissected from the explants and grown in murashing and skoog (ms) liquid medium. polymerase chain reaction (pcr) using the b gene and th...

In vitro the production of natural diosgenin from callus culture became an alternative biotechnological approach to production. This study describes effect plant growth regulators (PGRs) on induction and using different elicitors in induce (Diosgenin) parts Balanites Aegyptiacae extract cell lines. Root, stem, leaves calture B. aegyptiacae, were raised Murashing skoog Medium (MS) supplemented w...

Clerodendrum pholomidis is an important medicinal plant of Lamiaceae. Leaves, internode and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of 2, 4-dichlorophenoxy acetic acid (2, 4-D), kinetin (kn) and indole-3 butyric acid (IBA). Leaf explants formed clusters of globular somatic embryos directly from epidermal cells within 12-15d when cultured ...

in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...