نتایج جستجو برای: one allele specific primers pair (cebasf/amyc5r)

تعداد نتایج: 3002150  

Journal: :علوم باغبانی ایران 0
علی رضا راحمی گروه علوم باغبانی دانشگاه آزاد، واحد علوم وتحقیقات، تهران محمدرضا فتاحی مقدم هیات علمی علی عبادی پردیس کشاورزی و منابع طبیعی دانشگاه تهران تکتم سادات تقوی پردیس کشاورزی و منابع طبیعی دانشگاه تهران داراب حسنی موسسه تحقیقات اصلاح و تهیه نهال و بذر، بخش باغبانی، کرج

almonds are self incompatible, the incompatibility being controlled by a multi allelic (s-alleles) single gene. the incompatibility alleles were studied in 96 samples of wild almonds and related prunus species from 10 taxonomic groups. polymerase chain reactions were carried out with six primer sets including: three degenerate primer pairs (paconsi-f(fam)/em-pc1consrd, paconsi-f(fam)/em-pc3cons...

Journal: :Plant physiology 2000
E Drenkard B G Richter S Rozen L M Stutius N A Angell M Mindrinos R J Cho P J Oefner R W Davis F M Ausubel

We developed a modified allele-specific PCR procedure for assaying single nucleotide polymorphisms (SNPs) and used the procedure (called SNAP for single-nucleotide amplified polymorphisms) to generate 62 Arabidopsis mapping markers. SNAP primers contain a single base pair mismatch within three nucleotides from the 3' end of one allele (the specific allele) and in addition have a 3' mismatch wit...

2015
Hideki Shojo Mayumi Tanaka Ryohei Takahashi Tsuneo Kakuda Noboru Adachi Ulrich Melcher

Polymerase chain reaction-amplified product length polymorphism (PCR-APLP) is one of the most convenient and reliable methods for single nucleotide polymorphism (SNP) analysis. This method is based on PCR, but uses allele-specific primers containing SNP sites at the 3'-terminus of each primer. To use this method at least two allele-specific primers and one "counter-primer", which serves as a co...

Behnam Derakhshani Mohammad Moghaddam Mohammad Reza Jalal Kamali Seyed Abolghasem Mohammadi

Wheat is a crop with spring and winter types and wide adaptability to different climate conditions. The wide adaptability of wheat is mainly controlled by three groups of genetic factors and among them vernalization (VRN) genes play pivotal role in determining spring and winter types. In this study, 395 Iranian wheat landraces were characterized with specific primer pairs designed based on VRN-...

Journal: :journal of agricultural science and technology 2012
s. rahimi kaldeh r. hosseini j. hajizadeh m. m. sohani

all species in the genus lysiphlebus förster (hymenoptera: braconidae, aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. a pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, l. fabarum marshall, collected from guilan province (iran)....

ذوالفقاری, محمد رضا, صدرنیا, مریم , مسببی, نوید,

Background and purpose: Molecular detection of antibiotic resistance in clinical strains of M.tuberculosis is of great importance. In this study, we developed a method for rapid detection of mutations resistant to the rifabutin antibiotic resistant gene. Material and methods: In this study 40 clinical isolates of M.tuberculosis including 12 resistant and 28 susceptible isolates to rifabutin we...

Journal: :SAARC Journal of Agriculture 2021

The molecular characterization of chilli germplasm was done based on estimation genetic diversity among the by using SSR markers. Forty germplasms were analyzed eight primers. primers produced 30 loci with an average value 3.75 alleles per locus. similarity index matrix ranged from zero to 2.74. Polymorphic information content (PIC) 0.543 0.735 0.658. highest number (five) allele observed in pr...

A. Rafat F. Ala Noshahr,

The families of TGF-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. Three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (GDF9), BMP15 and BMPR-IB have been shown to be essential for follicular growth and ovulation. Different mutations in the GDF9 gene ...

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