Background: The novel coronavirus SARS-CoV-2 has severely affected the health and economy of several countries. Multiple studies are in progress to design therapeutics against potential target proteins SARS-CoV-2, including 3CL protease, an essential protein for virus replication. Materials & methods: In this study we employed deep neural network-based generative predictive models de novo s...

As an important industrial enzyme, protease is widely used in feed, food and other fields. At present, the insufficient activity obtained from microorganisms cannot meet purpose of production. In this study, Bacillus amyloliquefaciens with high production was screened animal feces by plate transparent circle method. To improve protease, atmospheric room temperature plasma (ARTP) mutagenesis fir...

background: proteolytic enzymes have an important role in variety of physiological and pathological functions. they have been used in therapeutic and pharmaceutical applications. characterizations of extracellular proteases from various strains of s. marcescens indicate that most strains produce a very similar major metalloprotease. this metalloprotease (serrapeptidase, serrapeptase) is an impo...

Enzim protease merupakan kelompok enzim hidrolase yang menghidrolisis ikatan peptida menjadi oligopeptida dan asam amino. banyak dimanfaatkan adalah berasal dari bakteri. Penelitian ini melakukan penentuan kondisi optimum pertumbuhan bakteri, produksi, pemurnian karakterisasi pH, suhu, waktu inkubasi, pengaruh ion logam senyawa EDTA terhadap aktivitas enzim. Penentuan data kinetika termodinamik...

Since its outbreak, the severe acute respiratory syndrome—coronavirus 2 (SARS-CoV-2) has impacted quality of life and cost hundreds-of-thousands lives worldwide. Based on global spread mortality, there is an urgent need for novel treatments which can combat this disease. To date, 3-chymotrypsin-like protease (3CLpro), also known as main protease, considered among most important pharmacological ...

The human rhinovirus 14 3C protease (HRV3C protease), in fusion with glutathione S-transferase also referred to as PreScission™ protease, is a cysteine of particular interest for affinity tag removal from proteins due its stringent recognition sequence specificity (LEVLFQ/GX) and superior activity at low temperature. Here we report the expression, purification use construct HRV3C NT*-HRV3CP, th...

It has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. Now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (DD) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and R...

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...