Background: Type-1 diabetes (T1D) is an autoimmune disease in which T lymphocytes destroy insulin-producing β-cells. Control of self-reactive T lymphocytes and recovery of diabetic injury is the end point of T1D. Objective: To investigate generation of tolerogenic dendritic cells (tolDCs) as an innovative method of diabetes therapy. Methods: Lentivirus vector production was achieved by GIPZ mou...

prepulse inhibition (ppi), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. dopaminergic direct and indirect agonists impair ppi. however, the profile of dopaminergic receptors involved in ppi impairment by dopamine agonists is not clear. by injecting shrna expressing plasmids against dopamine d2 receptor genes (drd2) in the nucle...

aim : in this study, to clarify the smad4blocking impact on fibrosis process, we investigated its down-regulation by shrna on activated human lx-2 cell, in vitro.   background : liver fibrosis is a critical consequence of chronic damage to the liver that can progress toward advanced diseases, liver cirrhosis and hepatocellular carcinoma (hcc).  different smad proteins play as major mediators in...

Objective(s): ShRNA-mediated silencing strategy is considered to be a potent therapeutic approach. The present study aimed to assess the ability of the previously prepared polyethylenimine (PEI) derivative for the shRNA knock-down of the CD200 gene on the cells obtained from the patients with chronic lymphocytic leukemia (CLL). Materials and Methods: Since there are several investigations...

Objective(s): Non-invasive treatment options for retinoblastoma (RB), the most common malignant eye tumor among children, are lacking. Epithelial growth factor receptor (EGFR) accelerates cell proliferation, survival, and invasion of many tumors including RB. However, RB treatment by targeting EGFR has not yet been researched. In the current study, we investigated the effect of EGFR down-regula...