Restriction enzymes SpeI and XbaI were used in a pulsed-field gel electrophoresis (PFGE) study for molecular characterization of 146 clinical Burkholderia pseudomallei isolates. The PFGE parameters were optimized to enable comparable, reproducible, and robust results. The optimized parameters for both SpeI and XbaI restriction enzymes used in this study were 200 V and a pulse time of 5 to 65 s ...

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library an...

Drought forecasting plays a crucial role in drought mitigation actions. Thus, this research deals with linear stochastic models (autoregressive integrated moving average (ARIMA)) as a suitable tool to forecast drought. Several ARIMA models are developed for drought forecasting using the Standardized Precipitation Evapotranspiration Index (SPEI) in a hyper-arid climate. The results reveal that a...

Tree-ring samples from Chinese Pine (Pinus tabulaeformis Carr.) that were collected in the Taihe Mountains on the western Loess Plateau, China, were used to analyze the effects of climate and drought on radial growth and to reconstruct the mean April-June Standardized Precipitation Evapotranspiration Index (SPEI) during the period 1730-2012 AD. Precipitation positively affected tree growth prim...

The authors propose a new climatic drought index: the standardized precipitation evapotranspiration index (SPEI). The SPEI is based on precipitation and temperature data, and it has the advantage of combining multiscalar character with the capacity to include the effects of temperature variability on drought assessment. The procedure to calculate the index is detailed and involves a climatic wa...

The Ningxia Hui Autonomous Region of China (Ningxia) is an important food production area in northwest China severely affected by drought. The Standardized Precipitation Index (SPI) and Standardized Precipitation Evapotranspiration Index (SPEI) were calculated based on monthly meteorological data to explore climate change and variation in drought intensity, duration, frequency, and spatial exte...

drought indices are commonly used to quantify and assess drought characteristics. the standardized precipitation index (spi), and recently introduced standardized precipitation-evapotranspiration index (spei) are considered as universal meteorological drought indices which allow comparisons of drought conditions across different climate regions. the spi captures anomalies in precipitation, wher...

Grassland, as the primary vegetation on the Qinghai-Tibet Plateau, has been increasingly influenced by water availability due to climate change in last decades. Therefore, identifying the evolution of drought becomes crucial to the efficient management of grassland. However, it is not yet well understood as to the quantitative relationship between vegetation variations and drought at different ...

Global warming has resulted in increasingly frequent and severe drought and/or precipitation events. Severe drought limits crop water availability and impacts agricultural productivity and socioeconomic development. To quantify drought-induced yield loss during the main crop stages in Liaoning province, China, aspects of drought episodes (magnitude, duration, and frequency) were investigated du...