deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. dna-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy. in this study universal primers common to almost all fungi were used to amplification of internal transcribe spacer 1 and 2 region. subsequent restriction enzym...

Abstract: Despite several topical and systemic antifungal drugs are used for the treatment of fungal infection, Amphotericin B (AmB) is still one of the most common first-line choices in systemic fungal infection for over seventh decades from discovery. Amphotericin B which is belonged to the polyene group has a wide spectrum in vitro and in vivo antifungal activity. Its mechanism of antifunga...

To evaluate the efficacy of itraconazole capsules in prophylaxis for fungal infections in neutropenic patients, we conducted a prospective, double-blind, placebo-controlled, randomized trial. Patients with hematologic malignancies or those who received autologous bone marrow transplants were assigned either a regimen of itraconazole (100 mg orally twice daily; n=104) or of placebo (n=106). Over...

Advances in neonatal management have led to considerable improvement in newborn survival. However, early (<72 hours) and late (>72hours) onset systemic infections, both bacterial and fungal, remain a devastating complication and an important cause of morbidity and mortality in these babies. Most neonatal fungal infections are due to Candida species, particularly Candida albicans. The sources of...

The increased incidence of systemic fungal infections in the past two decades has been overwhelming. Earlier, it was pathogenic dimorphic fungi, which were known to cause systemic infections. However, starting from the 1960s, opportunistic fungi started causing more number of infections, especially in the immunocompromised host. More recently, newer and less common fungal agents are being incre...

A steady increase in the frequency of invasive fungal infections has been observed in the past 2 decades, particularly in immunosuppressed patients. In recipients of bone marrow transplants, Candida albicans and Aspergillus fumigatus remain the primary pathogens. In many centers, however, Candida species other than C albicans now predominate, and many cases of aspergillosis are due to species o...