Effect of Lipogel Containing Extracts of Eryngium campestre L. and Satureja hortensis L. on Wound Healing in Male Wistar Rats

  Background and purpose: Wound is a type of injury with high prevalence in the society. The main requirement for wound management is rapid and complete wound healing without the spread of infection. Medicinal plants are affordable and accessible and suggested as one of the key treatment options. The aim of this study was to investigate the effect of lipogel formulations containing the extracts of Eryngium campestre L. and Satureja hortensis L. on wound healing in Wistar rats. Materials and methods: In order to prepare the lipogel formula, several formulations were designed with different ratios of paraffin, polyethylene, and extracts which were all examined to find the best formulation. To this end, 20 rats were divided into four groups (n=5 per group), including normal saline, lipogel base, lipogel containing extracts, and active water. Full thickness wounds (2.5 * 2.5 cm) were made on the dorsal neck area. Wound size evaluation and histopathological study were done on days 3,5,7, and 12. Results: The formulation containing 95% paraffin and 5% polyethylene was selected as the final lipogel formulation due to better skin coverage, good consistency, and better uniformity. In vivo results showed significant differences in wound size between treatment group and other groups (P<0.05). According to histological studies, the rates of collagen deposition in groups treated with lipogel containing extracts and lipogel base were higher than other groups. Furthermore, fibroblast maturity was found to be significantly different between the rats treated with lipogel containing extracts and normal saline group (P< 0.05). Conclusion: This study showed that lipogel containing extracts of Eryngium campestre L. and Satureja hortensis L. was effective in full thickness wound healing.