P-74: Crocin Repopulate The Leydig and Sertoli Cells in Testis of Cyclophosphamide Treated Adult Mice

Background: Increases in the survival rate of men treated with chemotherapeutic drugs and their desire to have children precipitate concerns about the effects of these drugs on germ cells. This study performed to evaluation of protective effects of crocin in oxidative stress induced by cyclophosphamide (CP) on testis. Materials and Methods: Three groups (6 mice in each) of adult mice were used. Control group treated with normal saline via ip, and group 2 treated with CP 15 mg/kg/ week,ip, and group 3 treated with CP along with crocin 200 mg/kg/day,ip. After 35 days samples were took and fixed in 10% formal saline and paraffin sections were prepared and stained by H&E method. Mean distribution of Laydig and Sertoli cells were counted in 1 mm2 field in 5 regions of each slides by latticed objective device. All obtained data were analyzed by SPSS software in ANOVA and Duncan test. Results: Results showed that the mean distribution of Leydig and Sertoli cells in group that treated with CP (9.27 ± 0.34 and 21.86 ± 0.81), significantly was lower than control group (18.99 ± 0.64 and 61.72 ± 3.17), and mean distribution of Leydig and Sertoli cells (14.41 ± 0.37 and 26.19 ± 0.75) significantly were more than CP treated group (p<0.05). Conclusion: This study showed that crocin ameliorate the oxidative stress effects of CP on reproductive organ.