Supplemental Glutathione Improves Post-Thaw Quality of Holstein Bulls Sperm in a Nanomicelle based Extender
Adding antioxidants to semen extenders could reduce post-thaw sperm damages. Glutathione is an important intracellular antioxidant that has free radicals scavenging properties and multiple effects on DNA and protein synthesis. In the present study, effects different levels of reduced glutathione (GSH) containing 0 (control), 1.0, 2.5, or 5.0 mM in a nanomicelle-based extender on post-thaw quality of bovine sperm were investigated. Initially, lecithin was added to a tris-based extender containing GSH. The mixture was then emulsified by slowly shaking and sonification so that nano size of particles was obtained. Thirty-six ejaculates from six Holstein bulls were used. The post-thaw quality parameters including computer-assisted sperm analysis of motility, plasma membrane functionality, membrane integrity, acrosome integrity, morphology and level of apoptosis were measured. Compared to the control medium, the total motility and membrane integrity were significantly better in samples containing 2.5 mM GSH (67.3±1.2% vs. 73±1.2% and 71.3±1.5% vs. 77.6±1.5%, respectively). The extender supplemented with 2.5 mM GSH had the highest percentage of viable sperm during apoptotic test. In conclusion, supplementation of lecithin nanomicelle-based extender with 2.5 mM GSH reduced damages to bovine sperm during cryopreservation.
Effects of Centrifugation and Different Levels of Soybean Lecithin-Based Extender on Post-Thaw Sperm Quality of Ghezel Ram Semen
The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...متن کامل
Freezing stallion semen with the new Cáceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability.
Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...متن کامل
Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls
This study was conducted to explore the adding effect of manganese (Mn) to Tris extender on post cooling and cryopreservation semen attributes of Holstein bulls for different periods. Seven Holstein bulls of 3.5-4.5 years old were used in this study. Pooled semen was divided into three groups. The first group was diluted with Tris only (control group, G1). While the 2 nd (G2) and 3 rd (G3) grou...متن کامل
BACKGROUND The present study was conducted to evaluate seasonal variability in the quality of pre- and post-thaw semen parameters among sperm bank donors. METHODS The first two consecutive ejaculates during the months March (spring, 92 males), June (summer, 97 males), September (autumn, 81 males) and December (winter, 97 males) were analysed. A comparison was made between sperm parameters fro...متن کامل
Oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. Cadmium (Cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. Because of its long biological half-life (10–30 years), Cdaccumulates in the biological systems. The present study was designed to assess the concen...متن کامل
Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa.
In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...متن کامل
دوره 10 شماره 4
صفحات 615- 622
تاریخ انتشار 2020-12-01
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