Arezoo Tavakoli

Islamic azad university,Eghlid branch,Fars,Iran.

[ 1 ] - Expression, purification and kinetic characterization of recombinant benzoate dioxygenase from Rhodococcus ruber UKMP-5M

In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector.The recombinant plasmid pGEMT-benA was digested by d...

[ 2 ] - Biochemical characterization of recombinant benzyl alcohol dehydrogenase from Rhodococcus ruber UKMP-5M

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characteri...

[ 3 ] - Biochemical characterization of recombinant benzyl alcohol dehydrogenase from Rhodococcus ruber UKMP-5M

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characteri...

[ 4 ] - تاثیر عنصر روی بر عملکرد الکل دهیدروژناز

سابقه و هدف: الکل دهیدروژنازها (ADHs) جزو خانواده اکسیدوردکتازها هستند که می‌توانند الکل را به آلدهید تبدیل کنند. واحدهای ساختمانی موجود در الکل دهیدروژناز در دو گروه اصلی جایگاه کاتالیستی و جایگاه اتصال برای کوآنزیم قرار می‌گیرند. اتم روی ( Zn ) به بخش کاتالیستی متصل شده و دومین اتم +2 Zn در ساختمان پروتئین نقش دارد. هدف از این مطالعه بررسی تاثیر 2 Zn+ بر بنزیل الکل دهیدروژناز (BADH) در گونه‌ا...

[ 5 ] - بررسی فراوانی ناقلین استافیلوکوکوس آرئوس بینی و تعیین الگوی مقاومت آنتی بیوتیکی آن، مطالعه موردی دانشجویان پرستاری دانشگاه آزاد اسلامی واحد اقلید

زمینه و هدف: استافیلوکوکوس آرئوس یک عامل مهم در بروز عفونت‌های بیمارستانی است. برخی حامل این باکتری در بخش‌هایی از بدن مانند بینی هستند. ناقلین این باکتری در کارکنان بیمارستان‌ها مانند پرستاران بیشتر از معمول است و سپس سویه‌های استافیلوکوکوس آرئوس مقاوم به آنتی‌بیوتیک‌ها به بیماران منتقل می‌شوند. این مطالعه با هدف شناسایی و ارزیابی حاملین استافیلوکوکوس آرئوس در بینی و تعیین الگوی مقاومت آنتی‌بی...

[ 6 ] - Partial Purification and Characterization of the Recombinant Benzaldehyde Dehydrogenase from Rhodococcus ruber UKMP-5M

Background: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. Objectives: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized.Materials and Methods: The xylC was amplified and cloned in E. coli. The re...

[ 7 ] - Effect of coronavirus (Covid-19) pandemic on biological air pollutants: a case study of Valiasr hospital in Zanjan (2019-2020)

Background and Objective: Bioaerosols as small particles enter the body by inhalation and lead to respiratory diseases based on type, concentration, and exposure time. In sensitive workplaces such as medical centers, it is necessary to pay attention to the type and population of these pollutants and the possibility of nosocomial infections. In the present study, the population and type of bioae...

[ 8 ] - Evaluation of air pollutant concentrations and environmental parameters under normal condition and during novel Coronavirus pandemic, a case study in a hospital (2019-2020)

Background and Objective: Indoor air pollution in hospitals could be a serious health threat to the patients, medical staff, and visitors. In previous studies, the importance of paying attention to air quality during the Coronavirus pandemic has been proven. In this study, the effect of visiters' presence under normal conditions and the imposition of hospital restrictions at the time of pandemi...

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