Routine detection of AmpC beta lactamases in Escherichia coli is cumbersome. This study was performed to determine the usefulness of a modified disc diffusion test to detect AmpC beta lactamases in E.coli. 28 non-repetitive, cefoxitin resistant isolates of E.coli were collected from cases of urinary tract infections and screened phenotypically for ESBLs and AmpC beta lactamase using DDST (cefta...

The effects of blue light and calcium on elongation of hypocotyl segments of Cucumber (Cucumis sativa L. cv Burpee's Pickler) were studied. Cucumber seedlings grown in dim red light showed a rapid decline in the rate of hypocotyl elongation when irradiated with high intensity (100 micromoles per square meter per second) blue light. In intact, 4-day-old seedlings the inhibition began within 2 mi...

Acid azo dyes, most of them naphtholdisulfonic acid derivatives, were given intraperitoneally to rats and their effect on "alkaline" ribonuclease activity was studied in total homogenates of kidney cortex and liver. Acid treatment was used to release bound enzyme activity. Several of the dyes, including trypan blue, increased RNase activity in both organs 3 days after administration of single d...

The aim of this study was to investigate the effect of storage at -4 0 C on the ascorbic acid (vitamin C) content of grapes (Vitis vinifera) for 1, 2, 5 and 8 months. A sensitive UV-VIS spectrometric method based on Prussian Blue reaction was applied for vitamin C determination and some kinetic calculations were presented. The varieties of red and white grapes used were from Murfatlar vineyard:...

Mutation of a gatekeeper residue, tryptophan 37, in E. coli lipoic acid ligase (LplA), expands substrate specificity such that unnatural probes much larger than lipoic acid can be recognized. This approach, however, has not been successful for anionic substrates. An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligas...

A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In additio...

this study was designed to explore the protective effects of ascorbic acid and water extract of spirulina platensis (blue green algae) on methotrexate-induced lipid peroxidation using goat liver as the lipid source. this in vitro evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of tissue homogenates. the study reveals the li...