Human and bovine glomerular basement membranes (GBM) were previously shown to be a three-dimensional molecular sieve composed of pores and strands by negative staining and electron microscopy. In this study, rat GBM were isolated under several different conditions to rule out morphological changes due to isolation procedures. Rat GBM isolated under different conditions all showed the same morph...

Diagnostic electron microscopy for infectious diseases has the advantage that "everything" in the specimen can be observed, without a priori knowledge of the likely identity of the microorganisms present in the sample. The classical specimen preparation method used employs a droplet of sample, which allows particles to adsorb to a support film, and is subsequently negative stained. This "grid o...

The cores of a small proportion of the particles in purified preparations of type C viruses are characterized by two concentric shells that have a "double-ring" appearance in specimens prepared for electron microscopy by negative staining or thin sectioning techniques. This distinctive feature allows such particles to be recognized as viral in impure preparations. Detectability of double-ring p...

Standard methods of virus diagnosis may take many days to complete. As antiviral drugs are being used with more effectiveness, it becomes more important to develop rapid diagnostic methods. It takes only a few minutes to prepare and examine a specimen for electron microscopy (EM), using the negative staining technique. Viruses in the specimen can readily be identified by their morphology. In or...

AIM The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatment are necessary for mentioned cases. Usual diagnostic method for this parasite is fecal smear p...

AIMS to determine whether potential correlations between CD117 and PDGFRA might serve as an indication for targeted therapies. MATERIAL AND METHODS immunohistochemical expression of CD117 and PDGFRA was evaluated in 99 paraffin-embedded GISTs in conjunction with KIT and PDGFRA mutational status. RESULTS CD117-positive staining was noted in 93 out of 99 cases. The predominant staining patter...