BACKGROUND In France, assisted reproductive technologies involving a hepatitis C virus (HCV)-infected man requires the cryopreservation of potentially infected semen (in order to establish the presence of HCV), hence the need for a safe and secure storage system. We evaluated the safety of high-security straws for the conservation of semen containing HCV RNA under routine conditions. METHODS ...

background: ‍‍cryopreservation has some detrimental impacts on sperms surface molecules. modification of the sperm surface molecules can affect on fertility rate. one of the important surface molecules are glycoconjugates. objective: the objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure. materials and methods: forty five h...

Abstract. Seeds or plant organs are usually used as the materials for the long-term cryopreservation. The aim of this study is to investigate the possibility of seed cryopreservation of Lilium ledebourii (Baker) Boiss. as an endemic and endangered species because of genetic erosion. To evaluate seed potentials for the cryopreservation, four treatments including vitrification, 30% glycerol, desi...

An existing cryopreservation method for liver slices applies 12% dimethylsulfoxide and rapid freezing. We found that cells in rat liver slices cryopreserved in this manner deteriorated rapidly upon culturing. To improve this cryopreservation method, we varied the dimethylsulfoxide concentration (0, 12, 18, and 30%), the cryopreservation medium (Williams medium E, fetal calf serum, and Universit...

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. ...