Proliferation kinetics and cell cycle parameters have been measured in exponentially growing and density-inhibited (daily medium changes) stable-plateau phase cultures of Chang human liver (LICH) cells. The mean duration of all phases of the cell cycle among the cycling population was approximately doubled in density-inhibited cultures. About 50% of the population was effectively noncycling but...

The propagation of neural precursors in culture is an essential tool for the study of the signaling matrix that regulates their proliferation, self-renewal, and generation of terminally differentiated progeny. Neural precursors can be expanded in vitro using both adherent and non-adherent culture protocols. The culture of fetal human neural precursors in the absence of serum as free-floating cl...

Recent advances in tissue engineering technology suggest its application in different medical fields, including periodontology. There are some reports of new non-enzymatic methods of isolating human gingival fibroblast for short-time cultivation in vitro to be used in autologous gingival augmentation. The aim of this study was to obtain a simple and established method of culturing human gingiva...

The aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in Sterlet, Acipenser ruthenus. Oocytes for the primary culture were obtained from the ovaries of a 6 years old Sterlet 729 g in weight and 47 cm in total length. The oocytes were in advanced vitellogenesis stage (PI >10). A part of the ovary (containing about 300 follicles) w...

Background & Aim: Persian shallot (Allium hirtifolium Boiss) with common name of "Musir" is one of the endemic medicinal plants in Iran. Leaves and bulbs with antibacterial, antifungal, anticancer, anti-diabetes and anti-obesity effects are edible parts of this plant. The tissue culture is an influential method to multiply plant productions in order to meet demands of ...

Background & Aim: Effects of ultra violet spectrum on plants due to their obligatory requirement for sunlight for survival are unavoidable. Direct effects of UV on plant growth and development are generally negative and usually they use many different defense mechanisms to acclimate and protect themselves. Material & Methods: The present research for the first time was conducted to investigate...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...