Background: Similar to inflammatory cells, peripheral blood mononuclear cells (PBMCs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. In this study we determined the viability rate and response to phytohemagglutinin-A (PHA) of human PBMCs in normal urine. Methods: A number of 1×106 ficoll-hypaque isolated PBMCs were dispensed in 1 ml normal urine...

today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

Drug resistance in Mycobacterium tuberculosis presents an enormous public health threat. It is typically defined as >1% of drug resistant colonies using the agar proportion method. Detecting small numbers of drug resistant Tb in a population, also known as heteroresistance, is challenging with current methodologies. Here we have utilized digital PCR to detect heteroresistance within M. tubercul...

Molecular diagnostics has changed the way lung cancer patients are treated worldwide. Of several different testing methods available, PCR followed by directed sequencing and amplification refractory mutation system (ARMS) are the two most commonly used diagnostic methods worldwide to detect mutations at exon 2 and kinase domain exons 18-21 in lung KRAS EGFR cancer. Compared to ARMS, the PCR fol...

African swine fever virus (ASFV) is a highly pathogenic DNA virus that is the causative agent of African swine fever (ASF), an infectious disease of domestic and wild pigs of all breeds and ages, causing a range of syndromes. Acute disease is characterized by high fever, haemorrhages in the reticuloendothelial system, and a high mortality rate. A powerful novel diagnostic assay based on the Lin...