Single-nucleotide polymorphism (SNP) genotyping is widely used in genetic association studies to characterize genetic factors underlying inherited traits. Despite many recent advances in high-throughput SNP genotyping, inexpensive and flexible methods with reasonable throughput levels are still needed. Real-time PCR methods for discovering and genotyping SNPs are becoming increasingly important...

lentiviral vectors (lvs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. however, the use of lentiviruses in studies requires an accurate titration technique.quantitative real-time pcr (qpcr) is a sensitive technique for the indication and quantitation of retrovirals particles. in this study, we used the qpcr for lentiviral vector titeration. th...

beta-thalassemia, by its high frequency and heterogenecity, constitues a real problem of health in iran. aboute 13 beta globin mutations encompass 70-90% of mutations spectrum in iran, the rest are rare or unknown. in this study six mutations of the codon ivsi-130(g-c), fr16 (-c), codon35 (-c), fr23/24(-g), codon8 (+g) and codon 20 (gtg-gag) were recognized and added to spectrom of beta globin ...

background: asthma is caused by the combination of different factors. current concepts of asthma pathogenesis emphasize on gene-environment interactions. mega-genome scanning projects revealed that different single nucleotide polymorphisms (snps) are related to asthma susceptibility. rs7216389-t is one of them that is related to childhood asthma and its effect on childhood asthma severity has b...

objectives: in present study we aimed to clone the lux i gene encoding n-acyl-homoserine synthase detected in biofilm forming clinical isolates of acinetobacter baumannii and study its expression in escherichia coli transformants. materials and methods: four a. baumannii hospital strains which demonstrated strong biofilm activity were selected in this investigation. the presence of lux i gene w...

purpose: the main purpose of this study was to evaluate the prevalence of herpes simplex virus 1 and 2 (hsv-1 and hsv-2), varicella-zoster virus and adenovirus associated with conjunctivitis in swab samples of patients who referred to eye specialist hospitals in tehran. methods: in this cross-sectional study, swab samples from patients with acute conjunctivitis during the first sixth months of ...