Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

problem of evil is something which is considered against existence of god or his attributes like being omnipotent and absolutely good. philosophers and theologians have tried to solve this problem by different ways. the solution which is accepted by muslim philosophers is the nothingness of evils. according to this opinion evils are not except nothing of essence or perfections of essence. in th...

it is presumed by certain historians and biographers that imam husayn left mecca for iraq changing his intention of hajj or major pilgrimage into a minor one. this is the dominant view among them. studying this issue, the present article deals with the traditions relevant to the subject in hadith collections. a thorough examination of historical accounts and reliance on authentic traditions lea...

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...