A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...

ACADEMIC DISSERTATION IN PLANT PATHOLOGY To be presented with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki for public criticism in Viikki, Auditorium B2, on June 30th, at 12 o'clock noon. HELSINKI 2000 2 Mäki-Valkama, T. Resistance to Potato virus Y (PVY) in potato cv. Pito transformed with the P1 gene of PVY. Doctoral thesis in plant pathology. 43 p. ...

Potato is an important food crop worldwide and grown in a large number of countries. As such, the under disease pressures need for selecting resistance genes during breeding programs essential. Of particular importance within Australia other parts world potyvirus, virus Y (PVY). In this paper, three commonly used PVY markers, M45, RYSC3 M6, were evaluated using existing genomic resources phenot...

Viruses pose a serious threat to the sustainable production of economically important crops around world. In past 20 years, potato virus Y (PVY) emerged as relatively new and very problem in potatoes, even though it is oldest known plant virus. Multiple strains cause various symptoms on leaves tubers resulting yield reduction poor-quality tubers. Consequently, would be interesting learn what ca...

Viral diseases have been a major limiting factor threating sustainable potato (Solanum tuberosum L.) production in Pakistan. Surveys were conducted to serologically quantify the incidence of RNA viruses infecting potato; Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato virus A (PVA), Potato virus M (PVM) and Potato leaf roll virus (PLRV) in two major potato cultivars (De...