نتایج جستجو برای: qualitative pcr
تعداد نتایج: 375243 فیلتر نتایج به سال:
AIMS Three pre-PCR processing strategies for the detection and/or quantification of Salmonella in naturally contaminated soya bean meal were evaluated. METHODS AND RESULTS Methods included: (i) flotation-qPCR [enumeration of intact Salmonella cells prior to quantitative PCR (qPCR)], (ii) MPN-PCR (modified most probable number method combined with qPCR) and (iii) qualitative culture enrichment...
In the present prospective study, five blood tests for detection of human cytomegalovirus (HCMV), nucleic acid sequence-based amplification (NASBA) for detection of early (immediate-early antigen) and late (pp67) mRNA, PCR for detection of HCMV DNA (DNA PCR), culture, and pp65 antigenemia assay, and culture and DNA PCR of urine and throat swab specimens were compared for their abilities to pred...
We investigate the effect of pair creation on a shock structure. Actually, particles accelerated by a shock can be sufficiently energetic to boost, via Inverse Compton (IC) process for example, surrounding soft photons above the rest mass electron energy and thus to trigger the pair creation process. The increase of the associated pair pressure is thus able to disrupt the plasma flow and possib...
BACKGROUND Despite the rapid transition into routine clinical practice of molecular techniques based on PCR, external quality assessment (EQA) is still not widely available. The European Union and European Communities Confederation of Clinical Chemistry have supported the EQUAL project as a series of 3 different EQA programs for the assessment of molecular methods independently from analytes. W...
Hybrid gene PML-RARα is the molecular target found in most cases of acute promyelocytic leukemia (APL) and has been used for diagnosis and minimal residual disease studies. The standard molecular technique employed is qualitative reverse transcriptase-polymerase chain reaction (RT-PCR), but with the emergence of real time PCR (Q-PCR), PML-RARα gene detection approaches have been described allow...
High risk human papillomaviruses (HR-HPVs) are associated with increased risk of normal cervical cells developing to dysplasia and cervical carcinoma. Therefore, HR-HPV DNA testing can predict an endpoint of cervical carcinogenesis that is earlier than the development of cervical abnormalities. Not only the sensitivity of methods but also the amount of HPV DNA are very important and might be pa...
1 There is a need for standardization and simplification of the existing methods for molecular 2 detection of Leishmania infantum in the canine reservoir host. The commercially available 3 OligoC-TesT kit incorporates standardized PCR reagents with rapid oligochromatographic 4 dipstick detection of PCR products, and is highly sensitive in humans, but not yet 5 independently validated in dogs. H...
A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤ 1% were found for all...
Introduction Breast duct lavage for analysis by methylation-specific PCR is a novel but established method for detecting the presence of cancer and may contribute additional information about prognosis and response to treatment. The aim of this consecutive series of breast cancer patients was a case-control study to evaluate qualitative methylation of five published tumour suppressor genes1 in ...
this research aims at answering the questions about translation problems and strategies applied by translators when translating cultural concepts. in order to address this issue, qualitative and quantitative study were conducted on two groups of subjects at imam reza international university of mashhad. these two groups were assigned as beginner and advanced translation students (10 students). ...
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