The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of Haelll, EcoRI and PstI restriction endonucleases has been investigated by electron microscopy analysis. The extent of joining is maxi_ mal at 4° and decreases with increasing temperatures following sigmoid like curves. The temperature at which 50?J of the maximal reaction is still observ...

RNA synthesis by the Azofobacter vinelandii RNA polymerase is initiated with the incorporation of a ribonucleoside triphosphate to form the 5’ terminus of the chain. The RNA chains synthesized in vifro are preferentially initiated by purine ribonucleoside triphosphates. The ratio of guanosine triphosphate to ATP termini is determined by the DNA used to direct the synthesis of RNA. When denature...

The synthesis of RNA molecules carrying lipids at their 3'-termini and 5'-termini is reported. These conjugates were fully characterized by MALDI-TOF mass spectrometry and HPLC chromatography. The ability of these conjugates to silence gene expression was evaluated in the inhibition of the tumor necrosis factor. All the lipid-siRNA derivatives were compatible with RNA interference machinery if ...

The RNA-dependent RNA polymerase of vesicular stomatitis virus (VSV), a nonsegmented negative-strand RNA virus, directs two discrete RNA synthetic processes, transcription and replication. Available evidence suggests that the two short extragenic regions at the genomic termini, the 3' leader (Le) and the complement of the 5' trailer (TrC), contain essential signals for these processes. We exami...