نتایج جستجو برای: full length sequence
تعداد نتایج: 939796 فیلتر نتایج به سال:
The full-length genome sequence of a human enterovirus 71 (EV71) strain (EV71/CZTN01/CHN/2017) was isolated from a throat swab from a child in Changzhou, China, in 2017. According to the phylogenetic analyses, the full-genome sequence in this study belongs to sub-subgenotype C4a.
the purpose of this study is to investigate and explore the causes of identity crisis as one of the debilitating consequences of colonial project after the withdrawal of colonial power. in order to do justice to the full-length severity of displacement that was the fruit of indentureship, v. s. naipauls west indian epic, a house for mr biswas, has been scrutinized with the main focus being the ...
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
An efficient strategy for large-scale high-throughput transposon-mediated sequencing of cDNA clones.
We describe an efficient high-throughput method for accurate DNA sequencing of entire cDNA clones. Developed as part of our involvement in the Mammalian Gene Collection full-length cDNA sequencing initiative, the method has been used and refined in our laboratory since September 2000. Amenable to large scale projects, we have used the method to generate >7 Mb of accurate sequence from 3695 cand...
background: the mesobuthus eupeus scorpion venoms are known to contain α-toxin peptides, many of which interfere with k+ ion channel function. these toxin peptides have important value in the pharmacology and physiology studies of specific k+ channel of cells. objectives: given the lack of information of the molecular biology of peptides in the toxin of iranian scorpions, the aim of this stud...
BACKGROUND Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the biological functions of coded proteins. However, previous approaches for sequencing cDNA clones were expensive or time-consuming, and therefore, a fast and efficient sequencing approach was demanded. ...
PfEMP1 are variant parasite antigens that are inserted on the surface of Plasmodium falciparum infected erythrocytes (IE). Through interactions with various host molecules, PfEMP1 mediate IE sequestration in tissues and play a key role in the pathology of severe malaria. PfEMP1 is encoded by a diverse multi-gene family called var. Previous studies have shown that that expression of specific sub...
background: the association between hepatitis b virus (hbv) mutations and hepatocarcinogenesis were reported in the literature. preference for g over c in the leading dna strand has been reported to account for the asymmetry in nucleotide (nt) composition. the aim of this study was to analyze the complete genome sequence and compositional asymmetry of hbv in different stages of hepatitis b. met...
The constructs pEGFP BIN1 full length (isoform 8, 454 aa) and pEGFP BAR+PI (aa 1-281) were obtained from P. De Camilli (Yale University, New York). cDNA corresponding to MTM1 sequence (603 aa) was cloned into pEGFP-C1 (Clontech, N-ter GFP fusion) by PCR amplification followed by BamH1 restriction and into pCS2+ vector ([1], untagged). cDNA corresponding to the full length, BAR+PI, BAR (aa 1-255...
Dihydropteridine reductase (DHPR; EC 1.6.99.7) catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin and is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. A cDNA for human DHPR was isolated from a human liver cDNA library in the vector lambda gt11 using a monospecific antibody against sheep DHPR. The nucleic acid sequence and amino acid s...
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