نتایج جستجو برای: real time detection
تعداد نتایج: 2592735 فیلتر نتایج به سال:
fluorescence chemical sensors for the highly sensitive and selective determination of pb2+ , hg2+, co2+ and fe3+ ions in aqueous solutions are described. the ion sensing system was prepared by incorporating lipophilic ligand (l) as a neutral ion-selective fluoroionophore in the plasticized pvc membrane containing sodium tetraphenylborate or potasium tetrakis (p-chlorophenyl) borate as a liphoph...
background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
زعفران به عنوان گرانترین ادویه جهان از کلالههای خشک گیاه crocus sativus به دست میآید و به طور عمده به عنوان رنگ و طعم دهنده در صنایع غذایی و به علت داشتن ترکیبات فعال در پزشکی کاربرد دارد. در این مطالعه تنوع ژنتیکی میان 28 ژنوتیپ زعفران که از مناطق مختلف ایران جمع آوری شده بود، با استفاده از نشانگر srap ارزیابی شد. 19 ترکیب آغازگری در مجموع 147 قطعه چندشکل با میانگین 74/7 قطعه و میانگین pic ب...
background and objectives: pneumonia with acinetobacter baumannii has a major therapeutic problem in health care settings. decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. the aim of this study was to develop a rapid and sensitive method for direct detection of a. baumannii from respiratory specimens. materials and methods : a taqman ...
Background: Currently, with increasing risk of influenza A virus epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and treatment of these viruses. The H274Y mutation is from the most important drug resistant factors in influenza A viruses. The aim of this study was detection of Oseltamivir...
Real time and accurate fault detection has attracted an increasing attention with a growing demand for higher operational efficiency and safety of industrial gas turbines as complex engineering systems. Current methods based on condition monitoring data have drawbacks in using both expert knowledge and quantitative information for detecting faults. On account of this reason, this paper proposes...
چکیده در بخش اول این پروژه دو روش میکرواستخخرا به نامهای میکرواسخخرا امولسیونی با کمک اموا فراصتتتو 1 و میکرواستتتخخرا مای – مای با کمک هوا 2 ، به عنوان دو روش میکرواستتتخخرا بدون حلال پخشکننده 3 ، برای اسخخرا برخی هیتدروکربتنهای آروماتیتک حلقوی 4 از نمونتههای آبتی بهکار گرفخه و پارامخرهای موثری همچون نوع حلال اسختتتتخرا کننده، حجم حلال اسخخرا کننده، میزان نمک، زمان تابش اموا فراصتو ، تددا...
Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
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