In the present study we show that recombinant bacterial CotA-laccase from Bacillus subtilis is able to decolourise, at alkaline pH and in the absence of redox mediators, a variety of structurally different synthetic dyes. The enzymatic biotransformation of the azo dye Sudan Orange G (SOG) was addressed in more detail following a multidisciplinary approach. Biotransformation proceeds in a broad ...

OBJECTIVE To estimate the degree of internal exposure to organochlorine pesticides (persistent organic pollutants, POP) among inhabitants of an open-air dump. METHODS This was an observational study with simultaneous measurement and control, in which the criterion for subjects was that they dwelled in an area contaminated by POP and the effect of such contamination was the concentration of su...

Enzymes displayed on the Bacillus subtilis spore coat have several features that are useful for biocatalysis. The enzyme is preimmobilized an inert surface of coat, which due to natural sporulation process. As a result, protein stability can be increased, and they resistant environmental changes. Next, would not lyse under extreme conditions, such as in organic solvents. Furthermore, easily rem...

1 Henrique Cota de Freitas, Pontifical Catholic University of Minas Gerais, Post -Graduation Program in Electrical Engineering Student, 500 Av. Dom Cabral Coração Eucarístico Belo Horizonte Minas Gerais, [email protected] 2 Carlos Augusto Paiva da Silva Martins, Pontifical Catholic University of Minas Gerais, Post -Graduation Program in Electrical Engineering Professor, 500 Av. Dom Cabral, Coraç...

We have determined a 35-kb sequence of the groESL-gutR-cotA (45 degrees-52 degrees) region of the Bacillus subtilis genome. In addition to the groESL, gutRB and cotA genes reported previously, we have newly identified 24 ORFs including gutA and fruC genes, encoding glucitol permease and fructokinase, respectively. The inherent restriction/modification system genes, hsdMR and hsdMM, were mapped ...

Vanessa de Fátima Bernardes; Luis Otávio de Miranda Cota; Fernando de Oliveira Costa; Ricardo Alves Mesquita; Ricardo Santiago Gomez; Maria Cássia Ferreira Aguiar DDS, MS, Graduate student, Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Brazil DDS, MS, PhD, Adjunct Professor, Department of Oral Surgery and Pathology, School of Dentistry, Feder...

Two mechanisms of inactivation of slow inward current through calcium channels in frog skeletal muscle have been described: voltage dependent inactivation similar to that of classical sodium channels (Cota et al. 1982, 1984; Stanfield 1977); and calcium ions depletion inside the T-tubules as a result of inward current flowing through the T-membrane. In the latter case the inactivation is curren...