Glucocorticoids (GCs) generally stimulate gene transcription via consensus glucocorticoid response elements (GREs) located in the promoter region. To identify the GRE in the rat tyrosine hydroxylase (TH) gene promoter, we transiently transfected PC12 cells with a 9-kilobase (kb) TH promoter-luciferase (Luc) construct. Dexamethasone (Dex) stimulated Luc activity, which was abolished by mifeprist...

Defective interfering (DI) RNAs are often associated with transmission-defective isolates of wound tumor virus (WTV), a plant virus member of the Reoviridae. We report here the cloning and characterization of WTV genome segment S5 [2613 base pairs (bp)] and three related DI RNAs (587-776 bp). Each DI RNA was generated by a simple internal deletion event that resulted in no sequence rearrangemen...

BACKGROUND Individuals differ in their blood pressure (BP) response to changes in dietary sodium (Na+) intake. It is possible that differences in BP responses to dietary Na+ are influenced by genes. METHODS A total of 35 older (62.9 +/- 1.2 years) hypertensive subjects had their mean arterial blood pressure (MABP) determined after 8 days of low (20 mmol/day) and high (200 mmol/day) Na+ intake...

Alu elements, the most abundant retrotransposed elements in human genome, are commonly located introns and affect alternative splicing. We previously showed that an intronic antisense element enhanced splicing a minigene model. The RNA experiment was performed using consisting of exons 9–11 ACAT1 with AluSx inserted into intron 10 pCAGGS vector. Here, we explored sequence affects downstream exo...

Background: β-thalassemia is one of the most widespread disease in the world, including Iran. In this study, we reported, for the first time, A 290-bp β-globin gene deletion in the south of Iran. Methods: Four individuals from three unrelated families with Arabic ethnic background were studied in Khuzestan Province. Red blood cell indices and hemoglobin analysis were carried out accor...

The Mitochondrial DNA 4977-bp Deletion in Patients with Colorectal Cancer: a Case-control Study Iran

Aims Chemokine receptor type 5 is recognized as the main co-receptor in HIV infection. Many studies have shown that deletion of 32-base pairs (bp) both alleles CCR5 leads to reduction or elimination expression this gene immune cells and natural resistance This study aims determine frequency delta 32 mutations healthy people, referring health center Gonabad City, Iran, focus on finding new treat...

compelling evidence suggests that the oxidative stress plays a key role in the pathophysiology of cardiovascular disease (cvd). superoxide dismutase (sod) enzymes play a major role in detoxification of reactive oxygen species and protection against oxidative stress. we examined the possible association between a 50-bp insertion/deletion in the sod1 promoter 1684-bp upstream of the sod1 atg with...