The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to SPF Litopenaeus vannamei in laboratory captivity condition, in connection with to PCR detection and viral genome sequence. The nested PCR product band was ranged between 296 to 910 bp and was found during entire period from the experimental studies of SPF L...

Fusarium spp. have emerged as major opportunistic fungal agents. Since new antifungal agents exhibit variable activity against Fusarium isolates depending on the species, rapid identification at the species level is required. Conventional culture methods are difficult, fastidious, and sometimes inconclusive. In this work, we sequenced a 440-bp fragment encoding the 28S rRNA from 33 Fusarium iso...

The genetic relationship among the Escherichia coli pathotypes was investigated. We used random amplified polymorphic DNA (RAPD) data for constructing a dendrogram of 73 strains of diarrheagenic E. coli. A phylogenetic tree encompassing 15 serotypes from different pathotypes was constructed using multilocus sequence typing data. Phylogram clusters were used for validating RAPD data on the clona...

Previous work has shown that multiple infections of apple trees by distinctly different strains of ‘Candidatus Phytoplasma mali’ are widespread. In the current study, pathological data of infected trees with single or multiple phytoplasma were analyzed and compared with molecular data based on a hflB gene of the infecting phytoplasmas. Single-strand conformation polymorphism and sequence analys...

Human Leukocyte Antigen (HLA) loci widely known for their role in generation of immune responses are often considered to be effective in reconstructing human phylogenies due to high degree of polymorphism and rarity of recombination observed at HLA loci. In the present study, we have made an attempt to substantiate the phylogenetic potential of HLA class II loci by analyzing DRB1, DQA1 and DQB1...

In the present study, we screened blood DNA samples obtained from cattle bred in Brazil (n=164) and Ghana (n=80) for Babesia bovis using a diagnostic PCR assay and found prevalences of 14.6% and 46.3%, respectively. Subsequently, the genetic diversity of B. bovis in Thailand, Brazil and Ghana was analyzed, based on the DNA sequence of merozoite surface antigen-1 (MSA-1). In Thailand, MSA-1 sequ...