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Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...
cognitive studies of translation process have recently been awarded a great deal of attention. there exists a psychological angle to almost all translation activities. the present study, thus, deals with analysing the relationships between iranian prospective translators tolerance for ambiguity (ta) and their willingness to translate (wtt). the research was conducted as a mixed methods study, d...
We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.
Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...
BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...
Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.
abstract the problems caused by the traditional system of criminal justice (which are based on remuneration and rehabilitation) such as, lack of attention to victim’s rights and position and society, underlie the emergence of restorative justice which had the victim-centered perspective, with respect to the role of the offender and participation of civil society. meanwhile, criminal mediation ...
a semi-empirical mathematical model for predicting physical part of ignition delay period in the combustion of direct - injection diesel engines with swirl is developed . this model based on a single droplet evaporation model . the governing equations , namely , equations of droplet motion , heat and mass transfer were solved simultaneously using a rung-kutta step by step unmerical method . the...
supervisors play an undeniable role in training teachers, before starting their professional experience by preparing them, at the initial years of their teaching by checking their work within the proper framework, and later on during their teaching by assessing their progress. but surprisingly, exploring their attributes, professional demands, and qualifications has remained a neglected theme i...
fahr’s disease is a rare syndrome characterized by symmetrical and bilateral intracranial calcifications. we report a 21-year-old male who presented with aggression, talkativeness, restlessness, and insomnia of recent onset. his neurological examination was normal. mental status examination and clinical investigation revealed mania. brain revealed symmetrical large areas and foci of calcificati...
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