Background: In this study we evaluate the effects of different doses of GDF9 with fixed dose of BMP15 on quality of in vitro maturated sheep oocytes. Materials and Methods: Good ovine Cumulus-oocyte complexes (COCs) were first transferred to Pre-IVM medium, and then to three IVM mediums supplemented with 10%v/v BMP15 and: 1) 175 ng/ml 2) 131 ng/ml or 3) 219 ng/ml GDF9. After maturation period, ...

The development of ovarian follicles is a unique instance of a morphogenesis process still occurring during adult life and resulting from the interactions between somatic and germ cells. In mammals, the initiation of follicular development from the pool of resting follicles is characterized by an increase in the oocyte size concomitant with the surrounding somatic cells proliferating to build a...

Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...

background: oocyte maturation and subsequent in vitro production (ivp) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. this study was designed to evaluate the effects of protein source (fetal bovine serum, fbs, and bovine seru...

Artificial insemination (AI) and embryo transfer (ET) play an important role in the genetic improvement of sheep herds. This review primarily describes the methods that have recently been researched and/or adapted to circumvent the problematic anatomy of the ovine cervix, which prevents the widespread application of transcervical AI and ET in this species. A mechanism of cervical ripening at pa...

Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...

Background: Present study aims to investigate if PMC of ovine oocytes in the presence of cilostamide a phosphodiesterase type 3 (PDE3) inhibitor can improve the shortcomings of conventional IVM system. Materials and Methods: Immature ovine oocytes were retrieved from pre antral follicles of abattoir ovaries by aspiration and after washing in Htcm + %10 FBS were immediately classified in four gr...

The goals of the present study were to develop a simple method for obtain highly purified pig sperm hyaluronidase (pHyase) and assess its activity, function, safety. In mammals, sperm-specific glycophosphatidylinositol (GPI)-anchored Hyase assists penetration through cumulus mass surrounding egg aids in dispersal oocyte complex. Recently, Purified bovine (bHyase) has been shown enhance therapeu...

Most aspects of reproductive function including spermatogenesis, oocyte growth and maturation, early embryonic development, fetal and placental growth, and lactation can be affected by thermal stress. Furthermore, it has been shown that oxidative stress involves in the pathology of thermal stress. Therefore, the aim of this study was to investigate the impacts of thermal stress on the ovine mat...