Background: Coronavirus disease 2019 (Covid-19) remains a pandemic with multiple challenges to confirm patient infectivity: lack of sufficient tests, accurate results, validated quality, and timeliness results. We hypothesize that rapid 15-minute Point-Of-Care serological test evaluate past infection complements diagnostic testing for Covid-19 significantly enhances availability. Method: A thre...

Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted for which Melanaphis sacchari the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated prevalence of accessions Sorghum bicolor another natural host virus. Two field experiments, one 2016 with 19 sorghum lines and other 2017 15 lines, were established at Belle G...

Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...

among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total o...

background: bordetella pertussis is the causative agent of pertussis. in brazil, laboratory diagnosis of pertussis is based on the culture. in 2010, was standardized the real-time pcr taqman® in routine diagnosis. objectives: the aim of this study was to evaluate the impact achieved with the introduction of rt-pcr for the routine diagnosis of pertussis and to compare with the results obtained f...