Quantitative proteome research is greatly promoted by high-resolution parallel format assays. A characterization of protein complexes based on binding forces offers an unparalleled dynamic range and allows for the effective discrimination of non-specific interactions. Here we present a DNA-based Molecular Force Assay to quantify protein-protein interactions, namely the bond between different va...

Rhododendron arboreum belong to the family Ericaceae is a medicinally and economically important wild edible tree species from Himalayan region. The fresh flowers of R. after harvesting were treated at different temperatures separately. study revealed that quick- drying process was found for maximum harnessing phenolics, flavonoids, flavanols, antioxidant activity (FRAP assay DPPH assay) protei...

Soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. However, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. Therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 MAPK) as the main therapeutic targets...

Protein aggregation is phenomenon wherein protein loses its native structure and aggregates due to the adaption of non-native conformation. Amyloid aggregation formed by the accumulation of various proteins causes many diseases in humans and other organisms. Antioxidants can prevent proteins aggregation. Pulicaria undulata  extract along with phenolic compounds can increase protein stability an...

Abstract Objectives The study aims to develop a stable malnourished experimental mice model resembling the human population for future studies. Materials and Methodology At weaning, female BALB/c are separated into two groups: one receiving low protein diet (LPD, 10% protein) other commercially available normal pellet (ND, 18% protein). Model development stability were assessed using body mass ...