Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

Popular media describe adverse effects of helicopter parents who provide intense support to grown children, but few studies have examined implications of such intense support. Grown children (N = 592, M age = 23.82 years, 53% female, 35% members of racial/ethnic minority groups) and their parents (n = 399, M age = 50.67 years, 52% female; 34% members of racial/ethnic minority groups) reported o...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

A 23-year old man was presented with multiple papules andnodules (25 lesions) on the trunk and proximal extremities(Figure 1). Two or three lesions were appeared at childhood andincreased to 25 lesions in early adulthood. There were variationsin size from several millimeters to 2 cm with spontaneous andinducible pain especially in exposure to the cold environment.Same lesions were present in hi...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

background : acromegaly, a condition which is associated with an acquired progressive disfigurement mainly involving the face and extremities, is a clinical syndrome which results from excessive production of growth hormone (gh) most commonly due to adenoma of the pituitary gland. acromegaly is a rare disease which due to its slow progression, is mostly diagnosed in late adulthood. bilateral sy...