conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

conclusions the activities studied by the production of pnp were determined by following the increase at 410 nm. the maximum pnp was produced at 30°c with an optical density of 10.62 in the presence of cytoplasmic expression of oph (pet21a-opd). consequently, our results suggest cytoplasmic expression system as an appropriate candidate with a high amount of oph in spite of inclusion body format...

conclusions this study indicated the contamination of lettuce by stec strains and its possible role as the source of infection. resistance to both tetracycline and ampicillin may be considered as an emergency alarm for a multidrug resistance of stec strains. results eight samples were positive for presence of stec strains, three contained stx1, five contained stx2, and one sample was positive f...

results in this study, high resistances were found to nalidixic acid (59.7%) and gentamicin (52.3%) but high susceptibilities were found to ciprofloxacin (59.3%) and ceftriaxone (36.3%). conclusions according to the results obtained in this study ciprofloxacin is an effective antibiotic in e. coli infections. objectives the current study aimed to investigate the resistance of escherichia coli a...

background infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. enteropathogenic escherichia coli (epec) may cause infantile diarrhoea among children in developing countries. objectives the aim of this study was to define the prevalence of epec strains in raw milk samples. materials and methods raw milk samples co...

Flavoenzymes are versatile biocatalysts containing either FAD or FMN as cofactor. FAD often binds to a Rossmann fold, while FMN prefers a TIM-barrel or flavodoxin-like fold. Proline dehydrogenase is denoted as an exception: it possesses a TIM barrel-like fold while binding FAD. Using a riboflavin auxotrophic Escherichia coli strain and maltose-binding protein as solubility tag, we produced the ...

We analyzed the prevalence of diarrheagenic Escherichia coli (DEC) markers and common phenotypes in 2,164 E. coli isolates from 282 DEC-positive samples. Enteropathogenic E. coli (EPEC) and enteroaggregative E. coli (EAEC) were very diverse and were not correlated with diarrhea. Enterotoxigenic E. coli (ETEC) estA and enterohemorrhagic E. coli (EHEC) belonged to a few phenotypes and were signif...

BACKGROUND Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli for...

The combination of supramolecular hydrogels formed by low molecular weight gelator self-assembly via noncovalent interactions within a scaffold derived from polyethylene glycol (PEG) affords an interesting approach to immobilize fully functional, isolated reporter bacteria in novel microwell arrays. The PEG-based scaffold serves as a stabilizing element and provides physical support for the sel...