1. Three sheep fitted with rumen cannulas were fed hourly a daily ration of 1000 g pelleted-grass cubes, and during four successive 2-week periods were intraruminally infused (0.45 l/d) with solutions containing sodium chloride (0.47 mol/d), sodium bicarbonate (0.47 mol/d), .ammonium chloride (0.47 mol/d) and sodium butyrate (0.47 mol/d). Each solution, except that for NaHCO,, was adjusted to p...

Assessment of the rumen fluid is an ancillary examination technique used in the clinical examination both of the individual bovine patient and at population level. The specific function and structure of the rumen makes ruminants different from other animals. Therefore, it logical to assess the rumen in detail when confronted with a bovine patient. Assessment of the rumen fluid is essential to a...

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides w...

Seventeen 350 lb (159 kg) Holstein steers were utilized to determine stability of rumen pH postmortem. Cattle were randomly assigned to two whole-corn ration treatment groups: 1) limit-fed (7.5 lb; 3.4 kg) or 2) fed ad libitum (9.5 lb; 4.3 kg). Calves were humanely euthanized, rumen fluid was collected, and pH measurements were taken at 0, 1, 2, 3, 6, 12, and 24 hours postmortem. A subset of fi...

This study was conducted to investigate the effects of different levels of peptide supplementation on rumen fermentation pattern, digestibility and microbial protein synthesis. Three rumen-cannulated Holstein dairy cows were used in a 3 × 3 Latin square experiment within 21 days period. The ruminal infusion of sodium caseinate (CN) was 0 (control), 50 and 100 g/d. Dry matter intake, milk yield ...

It has been known for decades that microbial fermentation within the rumen is critical to postnatal rumen epithelial growth and maturation in ruminants, but the underlying mechanism is largely unknown. In this study, we determined the effect of rumen fluid, which should contain all products from rumen fermentation, on growth of rumen epithelial cells in vitro. Addition of 10% rumen fluid from c...