نتایج جستجو برای: vitro maturation
تعداد نتایج: 455993 فیلتر نتایج به سال:
Background The utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. The aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. MaterialsAndMethods The study was performed in a private IVF center. 318 germinal vesicle (GV...
Introduction: In vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study was designed to assess the effect of heated human follicular fluid (hHFF), which is s...
Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature mouse oocytes treated with DEHP and embryo development. Materials and Methods: NMRI mouse strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 immature oocytes were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 12...
Introduction: Access to modern methods for increasing the percentage of in vitro human and animal mature oocytes can be useful in the treatment of some forms of human infertility as well as proliferation of many domestic and wild animals which generation is endangered. Effect of low- frequency electromagnetic fields on in vitro growth and maturation of mouse oocytes is recently considered as a ...
Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...
Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...
The aim of this study was to investigate the effect of different concentrations of conjugated linoleic acid (CLA) on in vitro maturation (IVM) and lipid metabolism-related genes in oocytes. In vitro maturation of oocyte was performed in the presence of control (0.0), 50, 100, and 200 µM CLA. Nuclear status and mRNA abundance of selected genes were evaluated following 24 h of IVM. Following the ...
introduction: in this study in vitro maturation, resumption of meiosis and development of immature mouse oocytes in the presence of cysteamine was evaluated. meterial and methods: immature oocytes recovered from nmri mouse strain (4-6 weeks) were categorized in three experimental and one control group. the control included 345 immature oocytes which were matured in mem medium contain 5% fcs. in...
oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...
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