OCTOBER 2005 P lasmid DNA production is becoming increasingly important as gene therapies make their way into clinical trials and eventually into the pharmaceutical marketplace. To date, most efforts toward process development have focused on downstream processing. However, the quality of a final product is ultimately determined by fermentation strategy. Through optimization of the growth envir...

Motivation: As the human genome project progresses and some microbial and eukaryotic genomes are recognized, numerous biotechnological processes have attracted increasing number of biologists, bioengineers and computer scientists recently. Biotechnological processes profoundly involve production and analysis of highthroughput experimental data. Numerous sequence libraries of DNA and protein str...

BACKGROUND The design of foot and ankle orthoses is currently limited by the methods used to fabricate the devices, particularly in terms of geometric freedom and potential to include innovative new features. Additive manufacturing (AM) technologies, where objects are constructed via a series of sub-millimetre layers of a substrate material, may present the opportunity to overcome these limitat...

The undergraduate electrical engineering curriculum at Embry-Riddle Aeronautical University includes a three-course sequence in electromagnetism, radio frequency systems, and the RF laboratory (taken concurrently with the RF class). These courses are designed to support novice students in attaining the final learning objective of the RF Lab: the design and understanding of a simple digital radi...

OBJECTIVES To design, manufacture and analyze custom implants with functional gradation in macrostructure for attachment of amputation prostheses. METHODS The external shape of the implant was designed by extracting geometrical data of canine cadavers from computed tomography (CT) scans to suit the bone cavity. Three generations of implant designs were developed and were optimized with the he...