نتایج جستجو برای: plasmid by the restriction enzymes then

تعداد نتایج: 17032277  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید مدنی آذربایجان - دانشکده علوم پایه 1392

let g=(v,e) be a graph with vertex set v and edge set e.for two vertices u,v of g ,the closed interval i[u,v] ,consists of u,v and all vertices lying in some u-v geodesic in g.if s is a set of vertices of g then i[s]is the union of all sets i[u,v]for u,v ? s. if i[s]=v(g) , then s is a geodetic set for g.the geodetic number g(g) is the minimum cardinality of geodetic set.the maximum cardinalit...

پایان نامه :0 1375

to explore the idea the investingation proposed, aimed at finding whether the performances of the population of iranians students studying english in an efl context are consistent in l1 and l2 writing taks and whether there is a cross-linguistic transfer in this respect. in this regard the subjects were instructed to write four compositions-two in english and two in farsi-which consisted of an ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تبریز 1390

building on previous studies on the effectiveness of different types of written corrective feedback, the present study aimed at investigating whether direct focused corrective feedback and direct unfocused corrective feedback produced any differential effects on the accurate use of english articles by efl learners across two different proficiency levels (low and high). in current study, the par...

Journal: :iranian journal of veterinary medicine 2014
ania ahani azari taghi zahraei salehi bahar nayeri fasaei omid madadgar masoud alebouyeh

background: among all common techniques in sitedirectedmutagenesis, λ red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. in thismethod, there is always the risk of dna linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. objectives:to overcome this, we constructeda recombinant vector to disrupt phop gene in salmonella...

BACKGROUND: Among all common techniques in sitedirectedmutagenesis, λ Red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. In thismethod, there is always the risk of DNA Linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. OBJECTIVES:To overcome this, we constructeda recombinant vector to disrupt phoP gene in Salmonella...

Journal: :iranian journal of parasitology 0
saloomeh shirali dept. of biology, faculty of basic science, ahvaz branch, islamic azad university, ahvaz, iran and dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran. hamidreza haddadzadeh dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran. mehdi mohebali dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, iran and center for research of endemic parasites of iran (crepi), tehran university of medical sciences, tehran, iran. bahram kazemi cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran and dept. of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran. narges amini dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.

background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تحصیلات تکمیلی صنعتی کرمان - پژوهشکده برق و کامپیوتر 1390

a phase-locked loop (pll) based frequency synthesizer is an important circuit that is used in many applications, especially in communication systems such as ethernet receivers, disk drive read/write channels, digital mobile receivers, high-speed memory interfaces, system clock recovery and wireless communication system. other than requiring good signal purity such as low phase noise and low spu...

پایان نامه :0 1374

the following null hypothesis was proposed: h : there is no significant difference between the use of semantically or communicatively translates scientific texts. to test the null hypothesis, a number of procedures were taken first, two passages were selected form soyrcebooks of food and nutrition industry and gardening deciplines. each, in turn, was following by a number of comprehension quest...

Journal: :BioTechniques 2003
Kagnow H Gebreyesus Roland A Owens

A difficulty that is encountered when attempting to insert a PCR-amplified product or DNA fragment of interest into a particular vector is the presence within the insert of one or more internal restriction endonuclease (RE) sites identical to those selected for the flanks of the insert. Our method circumvents this problem by partially protecting internal RE sites while flanking sites for the sa...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهرکرد - دانشکده ادبیات و علوم انسانی 1389

current studies in second language (l2) learning have revealed the positive role of corrective feedback (cf) in both oral and written forms in different language features. the present study was an attempt to investigate the effect of both direct and indirect written corrective feedback (wcf) on the use of grammatical collocations in l2 writing. the study also sought to examine whether the effec...

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