Rapid non-culture-dependent assays for identification of fungi quicken diagnosis and prompt treatment of invasive fungal disease. Fungal DNA extracts from pure cultures of the most frequently isolated fungal pathogens belonging to the Genera Aspergillus, Candida and Cryptococcus along with less common pathogenic Genera were amplified with the general fungal primer pair internal transcribed spac...

We read with great interest the article by Mendez et al. (2) published in a recent issue evaluating different PCR primers for cytomegalovirus (CMV) detection. As CMV PCR becomes a frequently used assay in the management of immunocompromised patients, such as transplant recipients and human immunodeficiency virus-infected individuals, an increasing number of laboratories are establishing in-hous...

Genomic DNA from five kindreds and two individuals with hereditary elliptocytosis [HE(4.1+)] and a partial deficiency of protein 4.1 [HE(4.1+)] was extracted and probed with a cDNA for protein 4.1. When using a fragment of the cDNA that encompassed the coding region of the gene, two restriction fragment length polymorphisms segregating with protein 4.1 deficiency were found in one kindred when ...

Licorice (Glycyrrhiza glabra L.) is a medicinal plant species valued in many countries Asia and Europe for its phytochemical characteristics. biodiversity becoming threatened nowadays Iran due to increasing demand drastic decline of natural habitats. Therefore, licorice domestication would be necessary the near future, molecular breeding help introduce genotypes suitable cultivation. The presen...

Polymorphisms. BamHI, EcoRI, and TaqI restriction fragment length polymorphisms at the bovine calpastatin locus. Source and Description of Probe. A 2.2-kb cDNA insert coding for Domains 2 through 4 plus a 3’ untranslated region of the bovine calpastatin gene was excised from the pBluescript vector (Stratagene, La Jolla, CA) with EcoRI and XhoI. Method of Detection. Nylon filters were prehybridi...