Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...

During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for expression tests. In this study, the TA-GC cloning method is proposed, as a new, simple and efficien...

In order to excavate the related resistant genes in kenaf root-knot nematodes and explore molecular mechanism of interaction between nematodes, based on obtained gene  NPR1 nematode from by transcriptome sequencing, PCR RACE technology were used clone full length  in kenaf, was named as HcNPR1 this study. The cDNA  to has a 2 058 bp, its open reading frame (ORF) is 1 776 bp (Chr:...

The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...

Introduction: Helicobacter pylori (H. pylori) infection has remained as a global health problem. Animal studies demonstrated the role of H. pylori oipA gene in the development of gastric cancer. The aim of this study was the cloning and expression of Helicobacter pylori oipA gene in a bicistronic vector harboring mice IL-18 gene. Materials and methods: The target gene encoding oipA was amp...

background and aims: dna constructs containing hcv antigens have become one of the vaccine candidates for induction of anti-hcv cellular and humoral immunity. in this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of ns3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...

objective: il18 is a cytokine that plays an important role in the t-cell-helper type 1 (th1) response and hence, plasmid-encoded il18 is considered as a potent genetic adjuvant for dna vaccine studies. in this study, a bicistronic eukaryotic plasmid capable of secreting a more stable mouse il18 (fused with fcγ2a fragment) was constructed and expression of this chimer cytokine was also assessed....

Background: Betatrophin, a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. However, its precise physiological role remains unclear. Here, we report the cloning, expression, and purification steps of mouse betatrophin in a prokaryotic system, followed by its structural analysis.    Methods: Specific cloning primers were...