background: icter is the most prevalent disease in newborns. although most of the newborns affiliated with this seem healthy in other aspects, there is always a fear for toxic complication of indirect hyperbilirobinemia in the central nervous system. if the level of indirect bilirobin increases in the plasma to a level, which denotes the probability of danger, phototherapy must be carried out i...

background : some characteristics of lighting sources such as color properties and ultraviolet emissions have important roles on visual and non-visual health effects of lighting. this study aimed to investigate the light emissions of some compact fluorescent lamps (cfls) and incandescent lamps commercially available to the iranian consumers. methods: sixty lamps included 48 single envelope cfls...

An improved method for separation and fluorescent analysis of coproporphyrin and uroporphyrin in urine was presented by Sobel, Cano, and Thiers [Clin. Chem. 20, 1397 (1974)]. Accurate determination of porphyrin concentrations by this method is impeded by the fluorescent background in urine samples, which varies from one specimen to another. By use of a straightforward procedure, zinc may be inc...

Based on an independent forward model in fluorescent tomography, a parallel reconstructed scheme for inhomogeneous mediums with unknown absorption property is proposed in this paper. The method considers the two diffusion equations as separately describing the propagation of excited light in tissues with and without fluorescent probes inside. Then the concentration of fluorophores is obtained d...

A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a "linear amplification". This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to label the product on only one strand. The products can be applied without prior purification directly to a gel...

Introduction: Coronary angiography is a Diagnostic-Therapeutic method involving ionizing radiation. This method causes to DNA damage with form double stranded breaks which is followed by the phosphorylation of the histone, H2AX. H2AX is a key factor in the repair process of damaged DNA which will accumulate to damage sites. In human cells, H2AX constitutes about 10% of the H2A ...

Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...

The aim of this study was to compare Fluorescent(auraminO rhodamine,AO)method ,Ziehl-Neelsen (ZN hot method)and kinyoun(cold method) staining techniques for the diagnosis of Mycobacterium tuberculosis. Materials And Methods: 186(on 3 consecutive days) sputum samples were collected from 62 suspected pulmonary tuberculosis patients, processed by petroffs method, and subjected to direct microscopy...

OBJECTIVE The assay of DNA mismatch repair (MMR) activity can be used as a biomarker for environmental condition detection and human disease diagnosis. Radioactive ³²P-endlabeled DNA containing mismatch is extensively used as the substrate for MMR activity analyses. The aim of the present study is to develop a simple non-radioactive, but equally specific and sensitive method for the MMR activit...