نتایج جستجو برای: hiseq second

تعداد نتایج: 616088  

Journal: :American journal of physical anthropology 2018
Maria A Nieves-Colón Andrew T Ozga William J Pestle Andrea Cucina Vera Tiesler Travis W Stanton Anne C Stone

OBJECTIVES The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi-tropical sites in Tanzania, Mex...

Journal: :The Journal of antimicrobial chemotherapy 2014
Mohammad Hamidian Johanna J Kenyon Kathryn E Holt Derek Pickard Ruth M Hall

OBJECTIVES To locate the acquired bla(OXA-23) carbapenem resistance gene in an Australian A. baumannii global clone 1 (GC1) isolate. METHODS The genome of the extensively antibiotic-resistant GC1 isolate A85 harbouring bla(OXA-23) in Tn2006 was sequenced using Illumina HiSeq, and the reads were used to generate a de novo assembly. PCR was used to assemble relevant contigs. Sequences were comp...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی دکتر علی شریعتی 1392

a large number of single research studies on the effects of strategy-based instruction (sbi) in teaching english as a foreign or second language has been conducted so far. however, the lack of a comprehensive meta-analysis targeting the effectiveness of english language sbi is observed. moreover, the findings of experimental studies regarding the context of the english language, proficiency lev...

Journal: :Marine Genomics 2021

This is the first report of a transcriptome assembly newly discovered hydrothermal vent mussel, Gigantidas vrijenhoeki (Bivalvia: Mytilidae), on Central Indian Ridge. was identified from material collected at Onnuri Vent Field (OVF) Ridge in 2018, and reported as new species, distinct another dominant Bathymodiolus marisindicus, 2020. We sequenced G. using Illumina HiSeq X System. De novo analy...

2016
Adam G. Clooney Fiona Fouhy Roy D. Sleator Aisling O’ Driscoll Catherine Stanton Paul D. Cotter Marcus J. Claesson Bryan A White

Rapid advancements in sequencing technologies along with falling costs present widespread opportunities for microbiome studies across a vast and diverse array of environments. These impressive technological developments have been accompanied by a considerable growth in the number of methodological variables, including sampling, storage, DNA extraction, primer pairs, sequencing technology, chemi...

2012
Marc T. J. Johnson Eric J. Carpenter Zhijian Tian Richard Bruskiewich Jason N. Burris Charlotte T. Carrigan Mark W. Chase Neil D. Clarke Sarah Covshoff Claude W. dePamphilis Patrick P. Edger Falicia Goh Sean Graham Stephan Greiner Julian M. Hibberd Ingrid Jordon-Thaden Toni M. Kutchan James Leebens-Mack Michael Melkonian Nicholas Miles Henrietta Myburg Jordan Patterson J. Chris Pires Paula Ralph Megan Rolf Rowan F. Sage Douglas Soltis Pamela Soltis Dennis Stevenson C. Neal Stewart Barbara Surek Christina J. M. Thomsen Juan Carlos Villarreal Xiaolei Wu Yong Zhang Michael K. Deyholos Gane Ka-Shu Wong

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combination of existing and newly developed methods to isolate total RNA from 1115 samples from 695 pla...

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