some possible effects of exogenous application of salicylic acid (sa) and bacillus subtilis on infection potential of root and stem rot disease and on induction of resistance against this pathogen were investigated in laboratory and as well under greenhouse conditions. in vitro, the effectiveness of sa concentrations (2-8mm) on growth of b. subtilis was observed as negative, while sa applicatio...

l-Phenylalanine ammonia-lyase (PAL) activity develops in excised bean axes after approximately 5 hours of incubation and reaches a maximum level after 14 hours of incubation. Light does not affect the development of activity, but puromycin, cycloheximide, actinomycin D, and 5-fluorouracil inhibit.During this period of incubation both d- and l-p-fluorophenylalanine stimulate fresh weight increas...

L-3,4-Dihydroxyphenylalanine (L-DOPA) is a known allelochemical exuded from the roots of velvet bean (Mucuna pruriens L. Fabaceae). In the current work, we analyzed the effects of L-DOPA on the growth, the activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and peroxidase (POD), and the contents of phenylalanine, tyrosine, and lignin in maize (Zea mays) roots. Three-d...

This study was conducted to investigate certain characteristics of the purified Phenylalanine ammonia lyase (PAL). The results revealed that molecular weight 180 kDa by gel filtration and Native-PAGE. optimal pH temperature for enzyme activity were 7 40 °C respectively. stable against values range 6-8. retained 79% its total after incubation one hour at 50 7% (lost 93%) 70 °C. entire completely...

Phenylketonuria is the most prevalent disorder caused by an inborn error in aminoacid metabolism. It results from mutations in the phenylalanine hydroxylase gene. Phenotypes can vary from a very mild increase in blood phenylalanine concentrations to a severe classic phenotype with pronounced hyperphenylalaninaemia, which, if untreated, results in profound and irreversible mental disability. Neo...

The purpose of this study was to investigate the response of physicochemical properties of gerbera (Gerbera Jamesonii L.) to silica nanoparticles (Nanoparticle-SiO2) and calcium chelate (Ca-Chelate) in nutrient solutions. A factorial experiment based on completely randomized design with two factors was conducted in four replicates. The first factor the concentration of Nanoparticle-SiO2 in nutr...

Aromatic amino acid ammonia-lyases and aromatic amino acid 2,3-aminomutases contain the post-translationally formed prosthetic 3,5-dihydro-4-methylidene-5H-imidazol-5-one (MIO) group. MIO enzymes catalyze the stereoselective synthesis of α- or β-amino acid enantiomers, making these chemical processes environmentally friendly and affordable. Characterization of novel inhibitors enables structura...

Aryl metabolite biosynthesis was studied in the white rot fungus Bjerkandera adusta cultivated in a liquid medium supplemented with L-phenylalanine. Aromatic compounds were analyzed by gas chromatography-mass spectrometry following addition of labelled precursors ((14)C- and (13)C-labelled L-phenylalanine), which did not interfere with fungal metabolism. The major aromatic compounds identified ...