A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage λpL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of λpL promoter under heat shock induction. Using the constructed vector,...

Dual hybridization of highly fluorescent conjugated polymers and highly luminescent nanocrystals (NCs) is developed and demonstrated in multiple combinations for controlled white light generation with high color rendering index (CRI) (>80) for the first time. The generated white light is tuned using layer-by-layer assembly of CdSe/ZnS core-shell NCs closely packed on polyfluorene, hybridized on...

purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...

The stringent response alarmone guanosine tetraphosphate (ppGpp) affects transcription from many promoters. ppGpp binds directly to the transcription enzyme of Escherichia coli, RNA polymerase. Analysis of the crystal structure of RNA polymerase with ppGpp suggested that binding of this nucleotide may result in some conformational or post-translational alterations to the enzyme. These changes m...

Solid state optical properties of fluorescent materials are important for many and electronic photonic devices such as organic light emitting diodes, frequency down-converters or luminescent solar concentrators. Perylene diimides (PDIs) represent one the most popular semiconductors which find application in those phonic device applications. In this study, photophysical two dibrominated PDI (DiB...

Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions. Escherichia coli RI (EcoRI) endonuclease is a well-known restriction enzyme that recognizes the symmetrical hexanucleotide sequence GAATTC on duplex DNA and ...

We introduce white light generation using CdSe/ZnS core–shell nanocrystals of single, dual, triple and quadruple combinations hybridized with InGaN/GaN LEDs. Such hybridization of different nanocrystal combinations provides the ability to conveniently adjust white light parameters including the tristimulus coordinates (x, y), correlated colour temperature (Tc) and colour rending index (Ra). We ...

A multi-resonance thermally activated delayed fluorescence (MR-TADF) dendrimer emitter and a related reference MR-TADF compound were designed, synthesized, characterized for use as narrowband emitters in solution-processed OLEDs. The 1 wt% doped films PMMA film revealed that the compounds MR-D1 MR-D2 showed green emission at λPL of 490 495 nm with FWHM 23 29 nm, respectively. 50 mCP still show ...

Near-infrared (NIR) dyes are sought after for their utility in light harvesting, bioimaging, and light-mediated therapies. Since long-wavelength photoluminescence typically involves extensive π-conjugated systems of double bonds aromatic rings, it is often assumed that NIR have to be large molecules require complex syntheses. We challenge this assumption by demonstrating facile incorporation te...